|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
08/02/2024 |
Actualizado : |
08/02/2024 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
TORRES, P.; BEYHAUT, E.; ALTIER, N.; FRESIA, P.; GARAYCOCHEA, S.; MARTIN, N.; REGO, N.; CRISPO, N.; LAGE, M.; ARROSPIDE, G.; SUNDBERG, G.; CUITIÑO, M.J.; ABREO, E. |
Afiliación : |
PABLO ANDRES TORRES ASTETE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELENA BEYHAUT GUTIERREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; SILVIA RAQUEL GARAYCOCHEA SOLSONA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; N. MARTIN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; N. REGO, Institut Pasteur Montevideo; N. CRISPO, Institut Pasteur Montevideo; M. LAGE, Lallemand Inc (Lage y Cía); G. ARROSPIDE, Lallemand Inc (CALISTER S.A); G. SUNDBERG, Lafoner S.A.; MARIA JOSE CUITIÑO DE VEGA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Phosphorus mineralizing Bacillus co-inoculated with rhizobia interact with phosphorus fertilization to improve soybeans yield and affect bacterial rhizospheric community. [abstract. |
Complemento del título : |
Theme 2 - Phosphorus acquisition by plants and microorganisms. Oral presentation. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
In: Michelini, D.; Garaycochea, S. (Eds.). 7th Phosphorus in Soils and Plants Symposium (PSP7). "Towards a sustainable phosphorus utilization in agroecosystems." Book of abstracts. PSP7, 3-7 October 2022, Montevideo, Uruguay. p.36. |
Idioma : |
Inglés |
Notas : |
FUENTE DE FINANCIAMIENTO: Proyecto - ALI_1_2014_1_5046, ANII. -- Published By: The organizing committee of the 7th Symposium on Phosphorus in Soils and Plants (PSP7)- National Agricultural Research Institute and School of Agronomy, Universidad de la República, Uruguay. |
Contenido : |
This study investigates the co-inoculation of soybeans with rhizobia and PMB Bacillus strains with and without addition of P fertilizer on plant P uptake and crop yield. |
Palabras claves : |
PLATAFORMA DE BIOINSUMOS - INIA; Soybean rhizosphere. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17489/1/Torres-PSP7-2022-p.36.pdf
|
Marc : |
LEADER 01515nam a2200289 a 4500 001 1064456 005 2024-02-08 008 2022 bl uuuu u01u1 u #d 100 1 $aTORRES, P. 245 $aPhosphorus mineralizing Bacillus co-inoculated with rhizobia interact with phosphorus fertilization to improve soybeans yield and affect bacterial rhizospheric community. [abstract.$h[electronic resource] 260 $aIn: Michelini, D.; Garaycochea, S. (Eds.). 7th Phosphorus in Soils and Plants Symposium (PSP7). "Towards a sustainable phosphorus utilization in agroecosystems." Book of abstracts. PSP7, 3-7 October 2022, Montevideo, Uruguay. p.36.$c2022 500 $aFUENTE DE FINANCIAMIENTO: Proyecto - ALI_1_2014_1_5046, ANII. -- Published By: The organizing committee of the 7th Symposium on Phosphorus in Soils and Plants (PSP7)- National Agricultural Research Institute and School of Agronomy, Universidad de la República, Uruguay. 520 $aThis study investigates the co-inoculation of soybeans with rhizobia and PMB Bacillus strains with and without addition of P fertilizer on plant P uptake and crop yield. 653 $aPLATAFORMA DE BIOINSUMOS - INIA 653 $aSoybean rhizosphere 700 1 $aBEYHAUT, E. 700 1 $aALTIER, N. 700 1 $aFRESIA, P. 700 1 $aGARAYCOCHEA, S. 700 1 $aMARTIN, N. 700 1 $aREGO, N. 700 1 $aCRISPO, N. 700 1 $aLAGE, M. 700 1 $aARROSPIDE, G. 700 1 $aSUNDBERG, G. 700 1 $aCUITIÑO, M.J. 700 1 $aABREO, E.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
05/12/2016 |
Actualizado : |
07/12/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
CARLSON, J. S.; GIANNITTI, F.; VALKIÜNAS, G.; TELL, L. A.; SNIPES, J.; WRIGHT, S.; CORNEL, A. J. |
Afiliación : |
JENNY S. CARLSON, Mosquito Control Research Laboratory, Department of Entomology and Nematology, Kearney Agriculture Center, University of California; FEDERICO GIANNITTI, University of Minnesota, Saint Paul, MN, USA; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; University of California, Davis, CA, USA; GEDIMINAS VALKIÜNAS, Nature Research Centre, Akademijos 2; LISA A. TELL, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California; JOY SNIPES, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California; STAN WRIGHT, Sacramento-Yolo Mosquito Vector and Control District; ANTHONY J. CORNEL, Mosquito Control Research Laboratory, Department of Entomology and Nematology, Kearney Agriculture Center, University of California; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California. |
Título : |
A method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays. |
Complemento del título : |
Methodology. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Malaria Journal, 2016, v.15 (5): Article number 1198. OPEN ACCESS |
ISSN : |
1475-2875 |
DOI : |
10.1186/s12936-016-1198-5 |
Idioma : |
Inglés |
Notas : |
Article history: Received 08 July 2015 // Accepted 01 March 2016 //First Online 11 March 2016. |
Contenido : |
ABSTRACT.
Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated intravenously into canaries and once infection was established, Culex stigmatosoma, Cx. pipiens and Cx. quinquefasciatus mosquitoes were then allowed to feed on the infected canaries to validate the efficacy of this method for mosquito vector competence assays. Results: Storage of Plasmodium spp. - infected donor blood at 4°C yielded viable parasites for 48 h. All five experimentally-infected canaries developed clinical signs and were infectious. Pathologic examination of three canaries that later died revealed splenic lesions typical of avian malaria infection. Mosquito infectivity assays demonstrated that Cx. stigmatosoma and Cx. pipiens were competent vectors for Plasmodium cathemerium. Conclusions: A simple method of collecting and preserving avian whole blood with malaria parasites of low parasitaemia (?0.0005 %) was developed that remained viable for further experimental bird and mosquito infectivity assays. This method allows researchers interested in conducting infectivity assays on target Plasmodium spp. to collect these parasites directly from nature with minimal impact on wild birds.
© 2016 Carlson et al. MenosABSTRACT.
Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated int... Presentar Todo |
Palabras claves : |
ANIMAL EXPERIMENT; AVIAN MALARIA; BIRD INOCULATION; BLOOD PRESERVATION; CULEX spp. VECTORS; EXPERIMENTAL INFECTION; PATHOLOGY; PLASMODIUM CATHEMERIUM. |
Thesagro : |
MODELOS ANIMALES. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12151/1/s12936-016-1198-5.pdf
https://malariajournal.biomedcentral.com/articles/10.1186/s12936-016-1198-5
|
Marc : |
LEADER 03718naa a2200337 a 4500 001 1056201 005 2018-12-07 008 2016 bl uuuu u00u1 u #d 022 $a1475-2875 024 7 $a10.1186/s12936-016-1198-5$2DOI 100 1 $aCARLSON, J. S. 245 $aA method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays.$h[electronic resource] 260 $c2016 500 $aArticle history: Received 08 July 2015 // Accepted 01 March 2016 //First Online 11 March 2016. 520 $aABSTRACT. Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated intravenously into canaries and once infection was established, Culex stigmatosoma, Cx. pipiens and Cx. quinquefasciatus mosquitoes were then allowed to feed on the infected canaries to validate the efficacy of this method for mosquito vector competence assays. Results: Storage of Plasmodium spp. - infected donor blood at 4°C yielded viable parasites for 48 h. All five experimentally-infected canaries developed clinical signs and were infectious. Pathologic examination of three canaries that later died revealed splenic lesions typical of avian malaria infection. Mosquito infectivity assays demonstrated that Cx. stigmatosoma and Cx. pipiens were competent vectors for Plasmodium cathemerium. Conclusions: A simple method of collecting and preserving avian whole blood with malaria parasites of low parasitaemia (?0.0005 %) was developed that remained viable for further experimental bird and mosquito infectivity assays. This method allows researchers interested in conducting infectivity assays on target Plasmodium spp. to collect these parasites directly from nature with minimal impact on wild birds. © 2016 Carlson et al. 650 $aMODELOS ANIMALES 653 $aANIMAL EXPERIMENT 653 $aAVIAN MALARIA 653 $aBIRD INOCULATION 653 $aBLOOD PRESERVATION 653 $aCULEX spp. VECTORS 653 $aEXPERIMENTAL INFECTION 653 $aPATHOLOGY 653 $aPLASMODIUM CATHEMERIUM 700 1 $aGIANNITTI, F. 700 1 $aVALKIÜNAS, G. 700 1 $aTELL, L. A. 700 1 $aSNIPES, J. 700 1 $aWRIGHT, S. 700 1 $aCORNEL, A. J. 773 $tMalaria Journal, 2016$gv.15 (5): Article number 1198. OPEN ACCESS
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|